Microwave-stimulated fixation for electron microscopy using a domestic microwave oven.
نویسندگان
چکیده
We investigated microwave-stimulated fixation of tissues for transmission electron microscopy using a domestic microwave oven operating at a frequency of 2.45 GHz with an output power of 500W. Microwave-stimulated fixation, in 4% glutaraldehyde, of fresh rat kidney, liver, heart and brain tissues was compared to conventional fixation. Human renal biopsies were similarly studied. Electron microscopy showed excellent ultrastructural preservation comparable to that obtained by conventional fixation. The optimal temperature range for microwave-stimulated fixation was found to lie between 50 degrees C and 55 degrees C. Our results indicate that microwave-stimulated fixation is a rapid and reproducible technique and can be effectively applied to routine diagnostic pathology.
منابع مشابه
Photoluminescent Properties of CoMoO4 Nanorods Quickly Synthesized and Annealed in a Domestic Microwave Oven
A simple way to prepare and -CoMoO4 nanorods is reported in this paper. CoMoO4·xH2O nanorod precursors were obtained using the microwave-assisted hydrothermal (MAH) method. By annealing the as-prepared CoMoO4·xH2O precursor at 600 ̊C for 10 min in a domestic microwave oven, and -CoMoO4 nanorods were prepared. These powders were analyzed by X-ray diffraction (XRD), field emission scanning ele...
متن کاملبررسی تاثیر شیوه خشک سازی بر خواص اپتیکی فیلم های نازک نانو ساختاری اکسید نیکل
The nanostructured nickel oxide thin films were prepared by dip coating sol – gel method. Three methods (drying with oven, IR and microwave) have used for drying the films. The effect of drying method on the optical, molecular, electrical, structural, and morphology properties of the films were studied by Uv-Visible spectrophotometry, Fourier Transform Infrared spectroscopy, Hall effect, X-ray ...
متن کاملتاثیر امواج نشتی اجاق مایکروویو بر وزن بدن، هورمون‌های تیرویید، کورتیزول و لیپیدهای سرم در موش سوری نابالغ
Background: High-frequency electromagnetic field (EMF) is generated by differentsources such as radar installations, radio and television transmitters, medical microwave diathermy device and domestic use of microwave ovens. Radiation leakage of microwave oven may be harmful for users. Kids who are residing near their mothers may expose to electromagnetic field. This study was carrie...
متن کاملEnergy filtering transmission electron microscopy immunocytochemistry and antigen retrieval of surface layer proteins from Tannerella forsythensis using microwave or autoclave heating with citraconic anhydride
Tannerella forsythensis (Bacteroides forsythus), an anaerobic Gram-negative species of bacteria that plays a role in the progression of periodontal disease, has a unique bacterial protein profile. It is characterized by two unique protein bands with molecular weights of more than 200 kDa. It also is known to have a typical surface layer (S-layer) consisting of regularly arrayed subunits outside...
متن کاملOne dimensional models for the microwave heating of moist foodstuffs
Microwave ovens are frequently used in domestic situations for the heating of chilled foodstuffs which are generally approximately 80% by weight water. Rapid, internal heating are some of the key benefits over conventional ovens. The food industry utilises these attributes in a number of ways most in particular with the introduction of microwave ready meals and convenience food. It is important...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Malaysian journal of pathology
دوره 12 1 شماره
صفحات -
تاریخ انتشار 1990